![]() #Caprine engineering skin#Pre-clinical study done in albino mice model showed within 14 days, all the wounds were completely cured, full thickness skin was regenerated without any significant inflammatory response. In vitro cell culture studies by seeding 3 T3 mouse fibroblast cells on the scaffold revealed excellent cell viability, proliferation rate and adhesion in the scaffold. FTIR analysis showed the shifting of NH stretching (3400–3600 cm−1), amide I band at 3427 cm− cm−1 and disappearance of the peaks of CH asymmetrical stretching (3000–2800 cm−1), amide II and amide III band, which indicate formation of amide linkage or other interaction between the SF protein and AGDM. Here, we found that SF-AGDM modified with low concentration of SF showed good porosity 78.56 ± 14.30% and pore size 74.69 ± 28.66 μm as similar to the AGDM. SLS analysis showed the molecular weight of SF was 10,000 Da. The grafts (AGDM and SF-AGDM) were evaluated for skin tissue regeneration by subjecting it through physical, chemical and biological characterization. In the present study, we have fabricated Silk Fibroin (SF) modified hybrid acellular goat-dermal matrix (SF-AGDM) by modifying the AGDM in different concentration SF― 5, 10, 15%, for enhancing the wound healing process. Worldwide the demand of skin-graft has been increasing day by day, for these different biomaterials and techniques have been used. In conclusion, it can be said that GTC may find its application as biomaterial in skin tissue engineering. Throughout the study, calf skin collagen (CSC) was used as standard for comparative evaluation. Finally, in vivo study in mice model revealed that GTC has low immunogenicity and it augments healing process significantly. Immuno-cytochemical analysis in conjugation with traction force microscopy further confirmed a superior focal adhesion complex mediated cell-substrate interaction in GTC. Biocompatibility study showed that GTC augmented cell adhesion, cell cycle progression and proliferation. Results revealed that GTC was comprised of type-I collagen. ![]() Physico-chemical characterization of 1% acetic acid extracted GTC was done by SDS-PAGE, amino-acid composition analysis, FT-IR and CD spectroscopy. This article delineates the isolation, physico-chemical characterization, biocompatibility study and wound healing application of acid soluble caprine (goat) tendon collagen (GTC). Keeping the aforementioned perspective in mind, we explored the possibility of using domestic goat available in the subcontinent as a potential source of collagen for tissue-engineering application. However, a risk of a spongiform encephalopathy epidemic has limited the use of collagen from these sources. ![]() ![]() Collagens presently used in tissue engineering are primarily of bovine or porcine origin. ![]()
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